vector plko 1gfp (Addgene inc)
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Addgene inc
vector plko 1gfp
Vector Plko 1gfp, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 175 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/shrna+plasmid+vector/pLKO%2E1+GFP+shRNA+(Plasmid+%2330323)/pm42864360-465-16-18
Average 95 stars, based on 175 article reviews
Vector Plko 1gfp, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 175 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/shrna+plasmid+vector/pLKO%2E1+GFP+shRNA+(Plasmid+%2330323)/pm42864360-465-16-18
Average 95 stars, based on 175 article reviews
vector plko 1gfp - by Bioz Stars,
2026-10
95/100 stars
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shRNA:Article Title: Prohibitin 2 mediates the mitochondrial protective effect of the whey-derived tetrapeptide Gly-Thr-Trp-Tyr. Article Snippet: .. These oligonucleotides were phosphorylated, annealed, and inserted into the Article Title: ELF3 drives glioblastoma multiforme progression through the regulation of MXRA8 expression. Article Snippet: To generate MXRA8-overexpressing (OE) stable U251 cells, the full-length human MXRA8 cDNA was cloned into the lentiviral expression vector pLVX-Puro (Clontech). .. To generate ELF3-knockdown stable cells, a specific short hairpin RNA (shRNA) sequences targeting ELF3 were cloned into the Article Title: Quantitative DIA-MS Uncovers Functional Impact of SMARCA4 Knockdown in Group 3 Medulloblastoma. Article Snippet: Medulloblastoma, a pediatric brain tumor, frequently features chromatin modifier mutations, including SMARCA4 loss in the aggressive Group 3 subgroup.. While SMARCA4 is considered a tumor suppressor, the functional impact of its loss on the oncogenic programs in Group 3 MB remains poorly understood.. Using doxycycline-inducible shRNA constructs in HD-MB03 cells (a MYC-amplified Group MB model) to achieve SMARCA4 knockdown, we applied quantitative mass spectrometry to profile the resulting proteomic changes. Article Title: Single-cell landscape reveals PLPP2 upregulation underlying radiotherapy resistance and immune microenvironment remodeling in pancreatic cancer. Article Snippet: .. Generation of stable PLPP2-knockdown cell lines Murine KPC cells were transduced with lentiviral particles encoding two independent PLPP2-targeting shRNAs (shPLPP2-1, shPLPP2-2) cloned into the Plasmid Preparation:Article Title: Prohibitin 2 mediates the mitochondrial protective effect of the whey-derived tetrapeptide Gly-Thr-Trp-Tyr. Article Snippet: .. These oligonucleotides were phosphorylated, annealed, and inserted into the Article Title: ELF3 drives glioblastoma multiforme progression through the regulation of MXRA8 expression. Article Snippet: To generate MXRA8-overexpressing (OE) stable U251 cells, the full-length human MXRA8 cDNA was cloned into the lentiviral expression vector pLVX-Puro (Clontech). .. To generate ELF3-knockdown stable cells, a specific short hairpin RNA (shRNA) sequences targeting ELF3 were cloned into the Article Title: Quantitative DIA-MS Uncovers Functional Impact of SMARCA4 Knockdown in Group 3 Medulloblastoma. Article Snippet: Medulloblastoma, a pediatric brain tumor, frequently features chromatin modifier mutations, including SMARCA4 loss in the aggressive Group 3 subgroup.. While SMARCA4 is considered a tumor suppressor, the functional impact of its loss on the oncogenic programs in Group 3 MB remains poorly understood.. Using doxycycline-inducible shRNA constructs in HD-MB03 cells (a MYC-amplified Group MB model) to achieve SMARCA4 knockdown, we applied quantitative mass spectrometry to profile the resulting proteomic changes. Article Title: In vivo CRISPR screens identify dual functions of DNMT3A in mediating tumor immune evasion. Article Snippet: .. Briefly, HEK293T cells were transiently co-transfected with either the shDNMT3A plasmid, shNFYA plasmid, or the empty Article Title: Whole-exome sequencing reveals the genetic landscape and polygenic susceptibility in 1241 patients with clinically suspected hemophagocytic lymphohistiocytosis Article Snippet: .. Forward and reverse primers were annealed to generate double-stranded DNA, which was cloned into the pLV( Clone Assay:Article Title: ELF3 drives glioblastoma multiforme progression through the regulation of MXRA8 expression. Article Snippet: To generate MXRA8-overexpressing (OE) stable U251 cells, the full-length human MXRA8 cDNA was cloned into the lentiviral expression vector pLVX-Puro (Clontech). .. To generate ELF3-knockdown stable cells, a specific short hairpin RNA (shRNA) sequences targeting ELF3 were cloned into the Article Title: Quantitative DIA-MS Uncovers Functional Impact of SMARCA4 Knockdown in Group 3 Medulloblastoma. Article Snippet: Medulloblastoma, a pediatric brain tumor, frequently features chromatin modifier mutations, including SMARCA4 loss in the aggressive Group 3 subgroup.. While SMARCA4 is considered a tumor suppressor, the functional impact of its loss on the oncogenic programs in Group 3 MB remains poorly understood.. Using doxycycline-inducible shRNA constructs in HD-MB03 cells (a MYC-amplified Group MB model) to achieve SMARCA4 knockdown, we applied quantitative mass spectrometry to profile the resulting proteomic changes. Article Title: Single-cell landscape reveals PLPP2 upregulation underlying radiotherapy resistance and immune microenvironment remodeling in pancreatic cancer. Article Snippet: .. Generation of stable PLPP2-knockdown cell lines Murine KPC cells were transduced with lentiviral particles encoding two independent PLPP2-targeting shRNAs (shPLPP2-1, shPLPP2-2) cloned into the Article Title: Whole-exome sequencing reveals the genetic landscape and polygenic susceptibility in 1241 patients with clinically suspected hemophagocytic lymphohistiocytosis Article Snippet: .. Forward and reverse primers were annealed to generate double-stranded DNA, which was cloned into the pLV( other:Article Title: Histone lactylation regulates macrophage polarization and metabolic homeostasis during hypoxic lung injury. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Brilliant Violet 421TM anti-mouse CD31 Biolegend Cat#102423; RRID: AB_10896110 PerCP/Cyanine5.5 anti-mouse CD45 Biolegend Cat#103131; RRID: AB_830644 PE/Cyanine7 anti-mouse CD127 (IL-7Rα) Biolegend Cat# 135013; RRID: AB_2629756 APC anti-mouse IL-10 Biolegend Cat# 505009; RRID: AB_2616726 PerCP/Cyanine5.5 anti-mouse CD326 (Ep-CAM) Biolegend Cat# 118219; RRID: AB_2721490 PE/Cyanine7 anti-mouse IL-33Rα(IL1RL1, ST2) Biolegend Cat# 145315; RRID: AB_2716221 Brilliant Violet 421TM anti-mouse/human KLRG1 (MAFA) Biolegend Cat# 138413; RRID: AB_2563741 Brilliant Violet 510TM anti-mouse CD45.2 Biolegend Cat# 109837; RRID: AB_2563838 Alexa Fluor 700 anti-mouse CD90.2 (Thy1.2) Biolegend Cat# 105319; RRID: AB_10612720 APC anti-mouse Ly-6C Biolegend Cat# 128015; RRID: AB_1732076 Zombie NIRTM Fixable Viability Kit Biolegend Cat# 423105 TruStain FcXTM anti-mouse CD16/32 Biolegend Cat# 101319; RRID: AB_1574975 Recombinant Mouse IL-4 (carrier-free) Biolegend Cat#574302 Recombinant Mouse IFN-γ(carrier-free) Biolegend Cat#575302 Recombinant Mouse IL-10 (carrier-free) Biolegend Cat#575804 Bacterial and virus strains E. coli DH5a Tsingke Cat#TSC-C14 E. coli BL21 New England Biolabs Cat#C2527 Chemicals, peptides, and recombinant proteins DMEM Hyclone Cat#SH30243.01 Phosphatase Inhibitor Cocktail I MedChemExpress Cat#HY-K0021 Phosphatase Inhibitor Cocktail II MedChemExpress Cat#HY-K0022 PrimeScript RT Master Mix TAKARA Cat#RR036B Trizol Invitrogen Cat#15596018 protease inhibitor cocktail Sigma-Aldrich Cat# 04693132001 phosphatase inhibitor cocktail Sigma-Aldrich Cat# 04906837001 Actinomycin D Sigma-Aldrich Cat# A1410 Phorbol-12-myristate-13-acetate (PMA) Sigma-Aldrich Cat# 19–144 Percoll GE Healthcare Cat# 17–0891-01 Protein A/G Agarose MedChemExpress Cat# HY-K0230 DMEM, high glucose Gibco Cat# 11965092 RPMI 1640 Gibco Cat# 21870092 Pierce anti-HA agarose Sigma-Aldrich Cat# A2095 Polyethylenimine Linear (PEI) Sigma-Aldrich Cat# P3143 Triton X-100 Sigma-Aldrich Cat# X100 DNase I Roche Cat# 10104159001 Puromycin InvivoGen Cat# ant-pr-1 MG132 TargetMol Cat# T2154 Collagenase IV Sigma-Aldrich Cat# C5138 HBSS, no calcium, no magnesium, no phenol red Gibco Cat# 14175095 L-Lactic acid Proteintech Cat# CM04835 PEG8000 - RNase free, ≥50%, high purity Aladdin Scientific Cat# 25322–68-3 1,6-hexanediol Sigma-Aldrich Cat#240117 FB-23–2 Selleck Cat#S8837 Ribonuclease R Epicentre Cat#RNR07250 Cyto-FastTM Fix/Perm Buffer Biolegend Cat#426803 Oligomycin A Sigma-Aldrich Cat# 75351 FCCP Sigma-Aldrich Cat# C2920 (Continued on next page) 18 Cell Reports 45, 118059, October 27, 2026 Transduction:Article Title: Single-cell landscape reveals PLPP2 upregulation underlying radiotherapy resistance and immune microenvironment remodeling in pancreatic cancer. Article Snippet: .. Generation of stable PLPP2-knockdown cell lines Murine KPC cells were transduced with lentiviral particles encoding two independent PLPP2-targeting shRNAs (shPLPP2-1, shPLPP2-2) cloned into the Selection:Article Title: Single-cell landscape reveals PLPP2 upregulation underlying radiotherapy resistance and immune microenvironment remodeling in pancreatic cancer. Article Snippet: .. Generation of stable PLPP2-knockdown cell lines Murine KPC cells were transduced with lentiviral particles encoding two independent PLPP2-targeting shRNAs (shPLPP2-1, shPLPP2-2) cloned into the |